RNA

Part:BBa_K3001023:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lsh crRNA targeting RNA Mango with T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a T7 promoter to allow in vitro transcription with a T7 polymerase. A double terminator containing a native E. coli terminator and the T7 terminator is also present to signal transcription termination.


Source

This part was designed by Laura Keffer-Wilkes based on Munich 2017's designs. Synthesized by IDT.

References